From the roots of Pulsatilla koreana, three monodesmosides(pulsatilia saponins A, B and D) and two bisdesmosides(pulsatilla saponins F and H) were isolated. The structure of these saponins have been determined as hederagenin 3-0-alpha-L-rhamnopyranosyl(1->2)-alpha-L-arabinopyranoside(A), hederagenin 3-0-beta-D-glucopyranosyl(1->4)-alpha-L-arabinopyranoside(B), hederagenin 3-0-alpha-L-rhamnopyranosyl (1->2)-[beta-D-glucopyranosyl(1->4)]-alpha-L-arabinopyranoside(D), 3-0-alpha-L-rhamnopyranosyl(1->2)-alpha-L-arabinopyranosyl hederagenin 28-0-alpha-L-rhamnopyranosyl(1->4)-beta-D-glucopyranosyl(1->6)-beta-D-glucopyranosyl ester (F) and 3-0-alpha-L-rhamnopyranosyl(1->2)-beta-D-glucopyranosyl(1->4)]-alpha-L-arabinopyranosyl hederagenin 28-0-alpha-L-rhamnopyranosyl(1->4)-beta-D-glucopyranosyl(1->6)-beta-D-glucopyranosyl ester(H) on the basis of chemical and spectral studies. Pulsatilia saponin B is the first report of its presence in plants but saponins A, D, F, and H have recently been isolated from the same genus P. cernua.
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